دورية
The response of the small intestine to vitamin D. Correlation between calcium-binding-protein production and increased calcium absorption
| العنوان: | The response of the small intestine to vitamin D. Correlation between calcium-binding-protein production and increased calcium absorption |
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| المؤلفون: | Emtage, J S, Lawson, D E M, Kodicek, E |
| المصدر: | Biochemical Journal; November 1974, Vol. 144 Issue: 2 p339-346, 8p |
| مستخلص: | 1. The synthesis of calcium-binding protein, a protein produced in the small intestine in response to vitamin D, was investigated with a view to determining whether calcium-binding-protein production could be correlated with the stimulation of calcium absorption by vitamin D. 2. A radioimmunological assay, which can quantitatively estimate calcium-binding-protein concentrations as low as 1μg/g wet wt., was used to detect the synthesis of soluble calcium-binding protein. 3. When used on intestinal supernatants from chicks dosed with vitamin D, calcium-binding protein was not detectable at 8h but was present after 12h at a concentration of 8.6μg/g wet wt.; in agreement with this an increase in calcium absorption due to vitamin D was detected at 12h but not at 8h. 4. The synthesis of calcium-binding protein was also monitored directly by making use of the ability of the iodinated antiserum to bind specifically to nascent calcium-binding protein chains on intestinal polyribosomes; in this way calcium-binding-protein synthesis could be detected 8h after dosage with vitamin D. Further, the binding reaction indicated a near linear increase in the calcium-binding-protein-synthesizing capacity over a 16h period. 5. From the amount of calcium-binding protein present 12 and 24h after vitamin D administration it is calculated that calcium-binding-protein mRNA is produced at approx. 1mol/min per intestinal cell. 6. It is concluded that the high correlation between the initiation of calcium-binding-protein synthesis and the stimulation of calcium absorption by vitamin D strengthens the proposal that calcium-binding protein plays an important role in calcium transport. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 02646021 14708728 |
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| DOI: | 10.1042/bj1440339 |