دورية
The complete amino acid sequence of yeast phosphoglycerate kinase
العنوان: | The complete amino acid sequence of yeast phosphoglycerate kinase |
---|---|
المؤلفون: | Perkins, R E, Conroy, S C, Dunbar, B, Fothergill, L A, Tuite, M F, Dobson, M J, Kingsman, S M, Kingsman, A J |
المصدر: | Biochemical Journal; April 1983, Vol. 211 Issue: 1 p199-218, 20p |
مستخلص: | The complete amino acid sequence of yeast phosphoglycerate kinase, comprising 415 residues, was determined. The sequence of residues 1-173 was deduced mainly from nucleotide sequence analysis of a series of overlapping fragments derived from the relevant portion of a 2.95-kilobase endonuclease-HindIII-digest fragment containing the yeast phosphoglycerate kinase gene. The sequence of residues 174-415 was deduced mainly from amino acid sequence analysis of three CNBr-cleavage fragments, and from peptides derived from these fragments after digestion by a number of proteolytic enzymes. Cleavage at the two tryptophan residues with o-iodosobenzoic acid was also used to isolate fragments suitable for amino acid sequence analysis. Determination of the complete sequence now allows a detailed interpretation of the existing high-resolution X-ray-crystallographic structure. The sequence -Ile-Ile-Gly-Gly-Gly- occurs twice in distant parts of the linear sequence (residues 232-236 and 367-371). Both these regions contribute to the nucleoside phosphate-binding site. A comparison of the sequence of yeast phosphoglycerate kinase reported here with the sequences of phosphoglycerate kinase from horse muscle and human erythrocytes shows that the yeast enzyme is 64% identical with the mammalian enzymes. The yeast has strikingly fewer methionine, cysteine and tryptophan residues. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 02646021 14708728 |
---|---|
DOI: | 10.1042/bj2110199 |