دورية
Evidence that lysosomes are not involved in the degradation of myofibrillar proteins in rat skeletal muscle
| العنوان: | Evidence that lysosomes are not involved in the degradation of myofibrillar proteins in rat skeletal muscle |
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| المؤلفون: | Lowell, B B, Ruderman, N B, Goodman, M N |
| المصدر: | Biochemical Journal; February 1986, Vol. 234 Issue: 1 p237-240, 4p |
| مستخلص: | To examine the role of lysosomes in the degradation of skeletal-muscle myofibrillar proteins, we measured the release of N tau-methylhistidine from perfused muscle of starved and fed rats in the presence or absence of agents that inhibit lysosomal proteinase activity. After 1 day of starvation, the release of N tau-methylhistidine by perfused muscle of 4-, 8- and 24-week-old rats increased by 322, 159 and 134% respectively. On the other hand, total protein breakdown, assessed by tyrosine release, increased by 62, 20 and 20% respectively. Inhibitors of lysosomal proteinases as well as high concentrations of insulin or amino acids failed to diminish the release of N tau-methylhistidine by perfused muscle of starved and fed rats, despite a 25-35% inhibition of total protein breakdown. The data strongly suggest that the complete breakdown of myofibrillar proteins occurs via a non-lysosomal pathway. They also suggest that total proteolysis, which primarily reflects non-myofibrillar protein breakdown, occurs at least in part within lysosomes. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 02646021 14708728 |
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| DOI: | 10.1042/bj2340237 |