دورية
Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane
| العنوان: | Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane |
|---|---|
| المؤلفون: | MONTI, Eugenio, BASSI, Maria T., PAPINI, Nadia, RIBONI, Mirko, MANZONI, Marta, VENERANDO, Bruno, CROCI, Gianluigi, PRETI, Augusto, BALLABIO, Andrea, TETTAMANTI, Guido, BORSANI, Giuseppe |
| المصدر: | Biochemical Journal; July 2000, Vol. 349 Issue: 1 p343-351, 9p |
| مستخلص: | Several mammalian sialidases have been described so far, suggesting the existence of numerous polypeptides with different tissue distributions, subcellular localizations and substrate specificities. Among these enzymes, plasma-membrane-associated sialidase(s) have a pivotal role in modulating the ganglioside content of the lipid bilayer, suggesting their involvement in the complex mechanisms governing cell-surface biological functions. Here we describe the identification and expression of a human plasma-membrane-associated sialidase, NEU3, isolated starting from an expressed sequence tag (EST) clone. The cDNA for this sialidase encodes a 428-residue protein containing a putative transmembrane helix, a YRIP (single-letter amino acid codes) motif and three Asp boxes characteristic of sialidases. The polypeptide shows high sequence identity (78%) with the membrane-associated sialidase recently purified and cloned from Bos taurus. Northern blot analysis showed a wide pattern of expression of the gene, in both adult and fetal human tissues. Transient expression in COS7 cells permitted the detection of a sialidase activity with high activity towards ganglioside substrates at a pH optimum of 3.8. Immunofluorescence staining of the transfected COS7 cells demonstrated the protein's localization in the plasma membrane. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 02646021 14708728 |
|---|---|
| DOI: | 10.1042/bj3490343 |