دورية
Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium bergheiANKA Strain in a Murine Model
| العنوان: | Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium bergheiANKA Strain in a Murine Model |
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| المؤلفون: | Fan, Zhi-gang, Li, Xiao, Fu, Hai-yi, Zhou, Li-min, Gong, Fei-li, Fang, Min |
| المصدر: | Current Medical Science; December 2019, Vol. 39 Issue: 6 p883-889, 7p |
| مستخلص: | Malaria remains a global health problem. The relationship between Plasmodium spp. and the gut microbiota as well as the impact of Plasmodium spp. on the gut microbiota in vertebrate hosts is unclear. The aim of the current study was to evaluate the effect of blood-stage Plasmodiumparasites on the gut microbiota of mice. The gut microbiota was analyzed by 16S rRNA sequencing and bioinformatic analyses at three stages. The gut microbiota changed during the three phases: the healthy stage, the infection stage, and the cure stage (on the 9th day after malarial elimination). Moreover, the gut microbiota of these infected animals did not recover after malaria infection. There were 254 operational taxonomic units (OTUs) across all three stages, and there were unique strains or OTUs at each stage of the experiment. The percentages of community abundance of 8 OTUs changed significantly (P<0.05). The dominant OTU in both the healthy mice and the mice with malaria was OTU265, while that in the cured mice was OTU234. In addition, the changes in OTU147 were the most noteworthy. Its percentage of community abundance varied greatly, with higher values during malaria than before malaria infection and after malaria elimination. These results indicated that the external environment influenced the gut microbiota after host C57BL/6 mice were infected with blood-stage P. bergheiANKA and that the same was true during and after elimination of blood-stage P. bergheiANKA. In addition, we could not isolate OTU147 for further study. This study identified gut microbiota components that were reconstructed after infection by and elimination of blood-stage P. bergheiANKA in host C57BL/6 mice, and this process was affected by P. bergheiANKA and the external environment of the host. |
| قاعدة البيانات: | Supplemental Index |
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Full Text Finder | تدمد: | 20965230 2523899X |
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| DOI: | 10.1007/s11596-019-2119-y |