Advances in the Biosynthesis of Pyranocoumarins: Isolation and 13C-Incorporation Analysis by High-Performance Liquid Chromatography–Ultraviolet–Solid-Phase Extraction–Nuclear Magnetic Resonance Data

التفاصيل البيبلوغرافية
العنوان: Advances in the Biosynthesis of Pyranocoumarins: Isolation and 13C-Incorporation Analysis by High-Performance Liquid Chromatography–Ultraviolet–Solid-Phase Extraction–Nuclear Magnetic Resonance Data
المؤلفون: Amaral, Jéssica C., da Silva, Michelli M., da Silva, M. Fátima G. F., Alves, Thayana C., Ferreira, A. Gilberto, Forim, Moacir R., Fernandes, João B., Pina, Edieidia S., Lopes, Adriana A., Pereira, Ana M. S., Novelli, Valdenice M.
المصدر: Journal of Natural Products; May 2020, Vol. 83 Issue: 5 p1409-1415, 7p
مستخلص: Citrus sinensisand Citrus limoniawere obtained by germination from seeds, and isotopic-labeling experiments using d-[1-13C]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography–ultraviolet–solid-phase extraction–nuclear magnetic resonance, data and the 13C enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of Xylella fastidiosa, which causes citrus variegated chlorosis in orange.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:01633864
15206025
DOI:10.1021/acs.jnatprod.9b00607