دورية
Enhancement of gibberellic acid production from Fusarium fujikuroiby mutation breeding and glycerol addition
العنوان: | Enhancement of gibberellic acid production from Fusarium fujikuroiby mutation breeding and glycerol addition |
---|---|
المؤلفون: | Peng, Xiao-Lun, Zhao, Wei-Jun, Wang, Yuan-Shan, Dai, Ke-Lei, Cen, Yu-Ke, Liu, Zhi-Qiang, Zheng, Yu-Guo |
المصدر: | 3 Biotech; July 2020, Vol. 10 Issue: 7 |
مستخلص: | Gibberellic acid (GA3) is a natural plant growth hormone that has been widely used in agriculture and horticulture. To obtain higher GA3producing strains, the method of screening the strains for resistance to simvastatin was used after treatment with nitrosoguanidine (NTG) and gamma rays. The rationale for the strategy was that mutants showing simvastatin resistance were likely to be high GA3producers, as their activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is relatively more effective. GA3yield of mutant S109 increased by 14.2% than that of the original strain. The GA3production ability in mutant S109 remained relatively stable after ten generations. With the addition of 0.4 g glycerol on the 5th day during the fermentation process in Erlenmeyer flask, maximum GA3production of 2.7 g/L was achieved by this mutant, exhibiting 28.6% increase compared with original strain. Furthermore, we also achieved 2.8 g/L GA3and had a 33.3% increase with addition 20 g glycerol on the 5th day during the fermentation process in a 5-L bioreactor. Our results indicated efficient GA3production could be achieved on the condition that the supply of glycerol at the suitable conditions. This study would lay a foundation for industrial production of GA3. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 2190572X 21905738 |
---|---|
DOI: | 10.1007/s13205-020-02303-4 |