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Lactobacillusand Pediococcusameliorate progression of non-alcoholic fatty liver disease through modulation of the gut microbiome

التفاصيل البيبلوغرافية
العنوان: Lactobacillusand Pediococcusameliorate progression of non-alcoholic fatty liver disease through modulation of the gut microbiome
المؤلفون: Lee, Na Young, Yoon, Sang Jun, Han, Dae Hee, Gupta, Haripriya, Youn, Gi Soo, Shin, Min Jea, Ham, Young Lim, Kwak, Min Jung, Kim, Byung Yong, Yu, Jeong Seok, Lee, Do Yup, Park, Tae-Sik, Park, Si-Hyun, Kim, Byoung Kook, Joung, Hyun Chae, Choi, In Suk, Hong, Ji Taek, Kim, Dong Joon, Han, Sang Hak, Suk, Ki Tae
المصدر: Gut Microbes; July 2020, Vol. 11 Issue: 4 p882-899, 18p
مستخلص: ABSTRACTTargeting the gut-liver axis by modulating the gut-microbiome can be a promising therapeutic approach in nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the effects of single species and a combination of Lactobacillusand Pediococcusin NAFLD mice model. Six-week male C57BL/6J mice were divided into 9 groups (n = 10/group; normal, Western diet, and 7 Western diet-strains [109CFU/g, 8 weeks]). The strains used were L. bulgaricus, L. casei, L. helveticus, P. pentosaceusKID7, and three combinations (1: L. casei+L. helveticus, 2: L. casei+L. helveticus+P. pentosaceusKID7, and 3: L. casei+L. helveticus+L. bulgaricus). Liver/Body weight ratio, serum and stool analysis, liver pathology, and metagenomics by 16S rRNA-sequencing were examined. In the liver/body ratio, L. bulgaricus(5.1 ± 0.5), L. helveticus(5.2 ± 0.4), P. pentosaceusKID7 (5.5 ± 0.5), and combination1 and 2 (4.2 ± 0.6 and 4.8 ± 0.7) showed significant reductions compared with Western (6.2 ± 0.6)(p < 0.001). In terms of cholesterol and steatosis/inflammation/NAFLD activity, all groups except for L. caseiwere associated with an improvement (p< .05). The elevated level of tumor necrosis factor-α/interleukin-1β (pg/ml) in Western (65.8 ± 7.9/163.8 ± 12.2) was found to be significantly reduced in L. bulgaricus(24.2 ± 1.0/58.9 ± 15.3), L. casei(35.6 ± 2.1/62.9 ± 6.0), L. helveticus(43.4 ± 3.2/53.6 ± 7.5), and P. pentosaceusKID7 (22.9 ± 3.4/59.7 ± 12.2)(p < 0.01). Cytokines were improved in the combination groups. In metagenomics, each strains revealed a different composition and elevated Firmicutes/Bacteroidetesratio in the western (47.1) was decreased in L. bulgaricus(14.5), L. helveticus(3.0), and P. pentosaceusKID7 (13.3). L. bulgaricus, L. casei, L. helveticus, and P. pentosaceusKID7 supplementation can improve NAFLD-progression by modulating gut-microbiome and inflammatory pathway.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:19490976
19490984
DOI:10.1080/19490976.2020.1712984