التفاصيل البيبلوغرافية
| العنوان: |
Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| المؤلفون: |
Fra-Bido, Sigrid, Walker, Simon A., Innocentin, Silvia, Linterman, Michelle A. |
| المصدر: |
STAR Protocols; 20210101, Issue: Preprints |
| مستخلص: |
Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues. |
| قاعدة البيانات: |
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