دورية
Modulation of red cell band 4.1 function by cAMP-dependent kinase and protein kinase C phosphorylation.
| العنوان: | Modulation of red cell band 4.1 function by cAMP-dependent kinase and protein kinase C phosphorylation. |
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| المؤلفون: | Ling, E, Danilov, Y N, Cohen, C M |
| المصدر: | Journal of Biological Chemistry; February 1988, Vol. 263 Issue: 5 p2209-2216, 8p |
| مستخلص: | Human erythrocyte protein 4.1 is phosphorylated in vivo by several protein kinases including protein kinase C and cAMP-dependent kinase. We have used cAMP-dependent kinase purified from red cells and protein kinase C purified from brain to test the effects of phosphorylation on band 4.1 function. In solution, each kinase catalyzed the incorporation of 1-4 mol of PO4/mol of band 4.1. Phosphorylation of band 4.1 by each kinase resulted in a significant (50-80%) reduction in the ability of band 4.1 to promote spectrin binding to F-actin. Direct measurement of spectrin-band 4.1 binding showed that phosphorylation by each kinase also caused dramatic reduction in this association. Phosphorylation of band 4.1 by each kinase for increasing time periods enabled us to demonstrate an approximately linear inverse relationship between PO4 incorporation into band 4.1 and spectrin binding. These results show that phosphorylation of band 4.1 by cAMP-dependent kinase and protein kinase C may be central to the regulation of red cell cytoskeletal organization and membrane mechanical properties. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219258 1083351X |
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| DOI: | 10.1016/S0021-9258(18)69192-6 |