دورية
The Phosphorylation State of CD3γ Influences T Cell Responsiveness and Controls T Cell Receptor Cycling*
| العنوان: | The Phosphorylation State of CD3γ Influences T Cell Responsiveness and Controls T Cell Receptor Cycling* |
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| المؤلفون: | Dietrich, Jes, Bäckström, Thomas, Lauritsen, Jens Peter H., Kastrup, Jesper, Christensen, Mette D., von Bülow, Fritz, Palmer, Ed, Geisler, Carsten |
| المصدر: | Journal of Biological Chemistry; September 1998, Vol. 273 Issue: 37 p24232-24238, 7p |
| مستخلص: | The T cell receptor (TCR) is internalized following activation of protein kinase C (PKC) via a leucine (Leu)-based motif in CD3γ. Some studies have indicated that the TCR is recycled back to the cell surface following PKC-mediated internalization. The functional state of recycled TCR and the mechanisms involved in the sorting events following PKC-induced internalization are not known. In this study, we demonstrated that following PKC-induced internalization, the TCR is recycled back to the cell surface in a functional state. TCR recycling was dependent on dephosphorylation of CD3γ, probably mediated by the serine/threonine protein phosphatase-2A, but independent on microtubules or actin polymerization. Furthermore, in contrast to ligand-mediated TCR sorting, recycling of the TCR was independent of the tyrosine phosphatase CD45 and the Src tyrosine kinases p56Lckand p59Fyn. Studies of mutated TCR and chimeric CD4-CD3γ molecules demonstrated that CD3γ did not contain a recycling signal in itself. In contrast, the only sorting information in CD3γ was the Leu-based motif that mediated lysosomal sorting of chimeric CD4-CD3γ molecules. Finally, we found a correlation between the phosphorylation state of CD3γ and T cell responsiveness. Based on these observations a physiological role of CD3γ and TCR cycling is proposed. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219258 1083351X |
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| DOI: | 10.1074/jbc.273.37.24232 |