دورية
Expression of Peroxisome Proliferator-activated Receptor PPARδ Promotes Induction of PPARγ and Adipocyte Differentiation in 3T3C2 Fibroblasts*
| العنوان: | Expression of Peroxisome Proliferator-activated Receptor PPARδ Promotes Induction of PPARγ and Adipocyte Differentiation in 3T3C2 Fibroblasts* |
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| المؤلفون: | Bastie, Claire, Holst, Dorte, Gaillard, Danielle, Jehl-Pietri, Chantal, Grimaldi, Paul A. |
| المصدر: | Journal of Biological Chemistry; July 1999, Vol. 274 Issue: 31 p21920-21925, 6p |
| مستخلص: | Nutritional long chain fatty acids control adipose tissue mass by regulating the number and the size of adipocytes. The molecular mechanisms implicated in this action of fatty acids remain poorly understood. It has been well established that peroxisome proliferator-activated receptor (PPAR) γ, activated by specific prostanoids, plays a central role in the control of adipocyte gene expression and terminal differentiation. Thus far, the role of PPARδ in the control of adipose tissue mass has remained unclear. Herein, we report the effects of ectopically expressed PPARδ on the control of adipose-related gene expression and adipogenesis of 3T3C2 fibroblasts. Treatment of PPARδ-expressing fibroblasts with fatty acids alone did not stimulate adipogenesis, whereas exposure of cells to a combination of fatty acids and PPARγ activators promoted lipid accumulation and expression of a typical adipocyte program. At the molecular level, activation of PPARδ by fatty acids induced transcription of the genes encoding fatty acid transporter, adipocyte lipid-binding protein, and PPARγ. Subsequent activation of PPARγ by specific agonists appeared to be required to promote terminal differentiation. These data demonstrate that PPARγ gene expression is under the control of PPARδ activated by fatty acids and could explain, at least partially, the adipogenic action of nutritional fatty acids. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219258 1083351X |
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| DOI: | 10.1074/jbc.274.31.21920 |