دورية
Interaction of Two Multifunctional Proteins
| العنوان: | Interaction of Two Multifunctional Proteins |
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| المؤلفون: | Shnyreva, Maria, Schullery, Daniel S., Suzuki, Hideaki, Higaki, Yuji, Bomsztyk, Karol |
| المصدر: | Journal of Biological Chemistry; May 2000, Vol. 275 Issue: 20 p15498-15503, 6p |
| مستخلص: | The heterogeneous nuclear ribonucleoprotein (hnRNP) K, a component of the hnRNP particles, appears to be involved in several steps of regulation of gene expression. To gain insight into mechanisms of K protein action, we performed two-hybrid screens using full-length hnRNP K as a bait. Several novel protein partners were identified, including Y-box-binding protein (YB-1), splicing factors 9G8 and SRp20, DNA-methyltransferase, hnRNP L, and hnRNP U. In vitrobinding studies and co-immunoprecipitation from cellular extracts provided evidence for direct interaction between hnRNP K and YB-1. Two distinct domains in YB-1 were responsible for binding to K protein. Each protein was able to transactivate transcription from a polypyrimidine-rich promoter; however, this effect was reduced when K and YB-1 proteins were coexpressed suggesting a functional interaction between these two proteins. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219258 1083351X |
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| DOI: | 10.1074/jbc.275.20.15498 |