دورية
Genomic analysis of polycarpellary rice (Oryza sativaL.) through whole genome resequencing
| العنوان: | Genomic analysis of polycarpellary rice (Oryza sativaL.) through whole genome resequencing |
|---|---|
| المؤلفون: | Das, Soumya Prakash, Jasrotia, Rahul Singh, Deb, Debal, Iquebal, Mir Asif, Jaiswal, Sarika, Dey, Narottam |
| المصدر: | Journal of Plant Biochemistry and Biotechnology; June 2021, Vol. 30 Issue: 2 p364-372, 9p |
| مستخلص: | There is a natural floral organ mutant of rice (var. Jugal) where the florets, popularly known as spikelet bear multiple carpels and produce multiple kernels in most of its grain. In our earlier work a detailed study has been done on its morpho-anatomical structure with allelic diversity and expression study of the major genetic loci associated with floral organ development. In present study high throughput whole genome sequencing was done which generated about of 3.7 million base pair genomic data for downstream analysis. The reads were about 101 bases long and mapped to the Oryza sativavar. Nipponbare as reference genome. Genome wide variant analysis detected 1,096,419 variants which included 943,033 SNPs and 153,386 InDels. A total of 24,920 non-synonymous SNPs were identified for 11,529 identified genes. Chromosome-wise distribution of uniquely mapped reads onto reference genome showed that maximum reads were mapped to 1st chromosome and least to 9th chromosome. 10th chromosome showed highest density of variations (about 325.6 per 100 kb genome sequence). Detailed sequence analysis of 23 floral organ developmental genes detected 419 potent variants where DL(Drooping Leaf) and OSH1(Oryza sativa Homeobox1) genes showed highest number (32) of variants; whereas, MADS21(Minichromosome Agamous Deficient Serum Factor21) gene have lowest number (5) of variants. The information generated in this study will enrich the genomics of floral organ development in indica rice and cereal crops in general. |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 09717811 09741275 |
|---|---|
| DOI: | 10.1007/s13562-020-00602-8 |