دورية
Proteomic Analysis of Anaplasma phagocytophilumduring Infection of Human Myeloid Cells Identifies a Protein That Is Pronouncedly Upregulated on the Infectious Dense-Cored Cell
| العنوان: | Proteomic Analysis of Anaplasma phagocytophilumduring Infection of Human Myeloid Cells Identifies a Protein That Is Pronouncedly Upregulated on the Infectious Dense-Cored Cell |
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| المؤلفون: | Troese, Matthew J., Kahlon, Amandeep, Ragland, Stephanie A., Ottens, Andrew K., Ojogun, Nore, Nelson, Kristina T., Walker, Naomi J., Borjesson, Dori L., Carlyon, Jason A. |
| المصدر: | Infection and Immunity; September 2011, Vol. 79 Issue: 11 p4696-4707, 12p |
| مستخلص: | ABSTRACTAnaplasma phagocytophilumis an obligate intracellular bacterium that invades neutrophils to cause the emerging infectious disease human granulocytic anaplasmosis. A. phagocytophilumundergoes a biphasic developmental cycle, transitioning between an infectious dense-cored cell (DC) and a noninfectious reticulate cell (RC). To gain insights into the organism's biology and pathogenesis during human myeloid cell infection, we conducted proteomic analyses on A. phagocytophilumorganisms purified from HL-60 cells. A total of 324 proteins were unambiguously identified, thereby verifying 23.7% of the predicted A. phagocytophilumproteome. Fifty-three identified proteins had been previously annotated as hypothetical or conserved hypothetical. The second most abundant gene product, after the well-studied major surface protein 2 (P44), was the hitherto hypothetical protein APH_1235. APH_1235 homologs are found in other Anaplasmaand Ehrlichiaspecies but not in other bacteria. The aph_1235RNA level is increased 70-fold in the DC form relative to that in the RC form. Transcriptional upregulation of and our ability to detect APH_1235 correlate with RC to DC transition, DC exit from host cells, and subsequent DC binding and entry during the next round of infection. Immunoelectron microscopy pronouncedly detects APH_1235 on DC organisms, while detection on RC bacteria minimally, at best, exceeds background. This work represents an extensive study of the A. phagocytophilumproteome, discerns the complement of proteins that is generated during survival within human myeloid cells, and identifies APH_1235 as the first known protein that is pronouncedly upregulated on the infectious DC form. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00199567 10985522 |
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| DOI: | 10.1128/IAI.05658-11 |