دورية
Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae
| العنوان: | Regulatory mutations affecting ornithine decarboxylase activity in Saccharomyces cerevisiae |
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| المؤلفون: | Cohn, M S, Tabor, C W, Tabor, H |
| المصدر: | Journal of Bacteriology; June 1980, Vol. 142 Issue: 3 p791-799, 9p |
| مستخلص: | We isolated several strains of Saccharomyces cerevisiae containing mutations mapping at a single chromosomal gene (spe10); these strains are defective in the decarboxylation of L-ornithine to form putrescine and consequently do not synthesize spermidine and spermine. The growth of one of these mutants was completely eliminated in a polyamine-deficient medium; the growth rate was restored to normal if putrescine, spermidine, or spermine was added. spe10 is not linked to spe2 (adenosylmethionine decarboxylase) or spe3 (putrescine aminopropyltransferase [spermidine synthease]). spe 10 is probably a regulatory gene rather than the structural gene for ornithine decarboxylase, since we isolated two different mutations which bypassed spe10 mutants; these were spe4, an unliked recessive mutation, and spe40, a dominant mutation linked to spe10. Both spe4 and spe40 mutants exhibited a deficiency of spermidine aminopropyltransferase (spermine synthase), but not of putrescine aminopropyltransferase. This suggests that ornithine decarboxylase activity is negatively controlled by the presence of spermidine aminopropyltransferase. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219193 10985530 |
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| DOI: | 10.1128/jb.142.3.791-799.1980 |