دورية
The Absence of (p)ppGpp Renders Initiation of Escherichia coliChromosomal DNA Synthesis Independent of Growth Rates
| العنوان: | The Absence of (p)ppGpp Renders Initiation of Escherichia coliChromosomal DNA Synthesis Independent of Growth Rates |
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| المؤلفون: | Fernández-Coll, Llorenç, Maciag-Dorszynska, Monika, Tailor, Krishma, Vadia, Stephen, Levin, Petra Anne, Szalewska-Palasz, Agnieszka, Cashel, Michael |
| المصدر: | mBio; April 2020, Vol. 11 Issue: 2 |
| مستخلص: | Bacterial cells regulate their own chromosomal DNA synthesis and cell division depending on the growth conditions, producing more DNA when growing in nutritionally rich media than in poor media (i.e., human gut versus water reservoir). The accumulation of the nucleotide analog (p)ppGpp is usually viewed as serving to warn cells of impending peril due to otherwise lethal sources of stress, which stops growth and inhibits DNA, RNA, and protein synthesis. This work importantly finds that small physiological changes in (p)ppGpp basal levels associated with slow balanced exponential growth incrementally inhibit the intricate process of initiation of chromosomal DNA synthesis. Without (p)ppGpp, initiations mimic the high rates present during fast growth. Here, we report that the effect of (p)ppGpp may be due to the regulation of the expression of gyrase, an important enzyme for the replication of DNA that is a current target of several antibiotics. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 21612129 21507511 |
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| DOI: | 10.1128/mBio.03223-19 |