دورية
DNA polymerase activities in growing cells infected with simian virus 40
| العنوان: | DNA polymerase activities in growing cells infected with simian virus 40 |
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| المؤلفون: | Méchali, M, Girard, M, de Recondo, A M |
| المصدر: | The Journal of Virology; July 1977, Vol. 23 Issue: 1 p117-125, 9p |
| مستخلص: | Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity of DNA polymerase-alpha increased five- to sixfold in the nuclear Triton wash and threefold in the nuclei and then remained enhanced only inside the nuclei. That of DNA polymerases-beta and gamma increased mostly in the nuclei after infection. These results suggest that DNA polymerase-alpha could be the major enzyme involved in SV40 DNA replication. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 0022538X 10985514 |
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| DOI: | 10.1128/jvi.23.1.117-125.1977 |