دورية
Biosynthesis of hepatitis B virus e antigen: directed mutagenesis of the putative aspartyl protease site
| العنوان: | Biosynthesis of hepatitis B virus e antigen: directed mutagenesis of the putative aspartyl protease site |
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| المؤلفون: | Jean-Jean, O, Salhi, S, Carlier, D, Elie, C, De Recondo, A M, Rossignol, J M |
| المصدر: | The Journal of Virology; December 1989, Vol. 63 Issue: 12 p5497-5500, 4p |
| مستخلص: | The C gene products of all mammalian hepadnaviruses contain a region with sequence similarities to the catalytic center of the aspartyl proteases. This region could have the capacity to cleave precore proteins, leading to the synthesis of e antigen. By site-directed mutagenesis on a plasmid containing the hepatitis B virus C gene, we have replaced either the Asp residue of the putative aspartyl protease catalytic center or an Asp residue located 3 amino acids upstream. Transient expression of the mutated hepatitis B virus C gene in human and mouse cells showed that none of these mutations prevented the secretion of an accurately processed HBe antigen. Thus, we demonstrated that the aspartyl protease responsible for e antigen precursor processing is not C gene encoded but is more likely to be a cellular enzyme. From these results, we suggest a model for the mechanism of e antigen synthesis. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 0022538X 10985514 |
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| DOI: | 10.1128/jvi.63.12.5497-5500.1989 |