دورية
Each of the conserved sequence elements flanking the cleavage site of mammalian histone pre-mRNAs has a distinct role in the 3'-end processing reaction
| العنوان: | Each of the conserved sequence elements flanking the cleavage site of mammalian histone pre-mRNAs has a distinct role in the 3'-end processing reaction |
|---|---|
| المؤلفون: | Mowry, K L, Oh, R, Steitz, J A |
| المصدر: | Molecular and Cellular Biology; July 1989, Vol. 9 Issue: 7 p3105-3108, 4p |
| مستخلص: | To study the substrate requirements for the histone 3'-end processing reaction of mammalian histone pre-mRNAs, we created a set of mutations in the sequences flanking the processing site of a mouse H3 gene. We found that deletion of the downstream purine-rich element hypothesized to interact with U7 small nuclear RNA abolishes in vitro 3'-end processing. Somewhat surprisingly, however, mutations in the hairpin loop element which destabilize or destroy the secondary structure reduce but do not abolish 3'-end processing. This is in apparent contrast to results obtained for the sea urchin system, where both sequence elements appear to be absolutely required for 3'-end formation. |
| قاعدة البيانات: | Supplemental Index |
PDF full text from Publisher | تدمد: | 02707306 10985549 |
|---|---|
| DOI: | 10.1128/mcb.9.7.3105-3108.1989 |