دورية
Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs
| العنوان: | Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs |
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| المؤلفون: | Parnaik, V K, Kennady, P K |
| المصدر: | Molecular and Cellular Biology; March 1990, Vol. 10 Issue: 3 p1287-1292, 6p |
| مستخلص: | A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min. |
| قاعدة البيانات: | Supplemental Index |
PDF full text from Publisher | تدمد: | 02707306 10985549 |
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| DOI: | 10.1128/mcb.10.3.1287-1292.1990 |