دورية
The Human SWI-SNF Complex Protein p270 Is an ARID Family Member with Non-Sequence-Specific DNA Binding Activity
| العنوان: | The Human SWI-SNF Complex Protein p270 Is an ARID Family Member with Non-Sequence-Specific DNA Binding Activity |
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| المؤلفون: | Dallas, Peter B., Pacchione, Stephen, Wilsker, Deborah, Bowrin, Valerie, Kobayashi, Ryuji, Moran, Elizabeth |
| المصدر: | Molecular and Cellular Biology; May 2000, Vol. 20 Issue: 9 p3137-3146, 10p |
| مستخلص: | ABSTRACTp270 is an integral member of human SWI-SNF complexes, first identified through its shared antigenic specificity with p300 and CREB binding protein. The deduced amino acid sequence of p270 reported here indicates that it is a member of an evolutionarily conserved family of proteins distinguished by the presence of a DNA binding motif termed ARID (AT-rich interactive domain). The ARID consensus and other structural features are common to both p270 and yeast SWI1, suggesting that p270 is a human counterpart of SWI1. The approximately 100-residue ARID sequence is present in a series of proteins strongly implicated in the regulation of cell growth, development, and tissue-specific gene expression. Although about a dozen ARID proteins can be identified from database searches, to date, only Bright (a regulator of B-cell-specific gene expression), dead ringer (a Drosophila melanogastergene product required for normal development), and MRF-2 (which represses expression from the cytomegalovirus enhancer) have been analyzed directly in regard to their DNA binding properties. Each binds preferentially to AT-rich sites. In contrast, p270 shows no sequence preference in its DNA binding activity, thereby demonstrating that AT-rich binding is not an intrinsic property of ARID domains and that ARID family proteins may be involved in a wider range of DNA interactions. |
| قاعدة البيانات: | Supplemental Index |
PDF full text from Publisher | تدمد: | 02707306 10985549 |
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| DOI: | 10.1128/MCB.20.9.3137-3146.2000 |