التفاصيل البيبلوغرافية
العنوان: |
Mammalian complex III heme dynamics studied with pump-probe spectroscopy and red light illuminations |
المؤلفون: |
O’Connor, Sean P., Powell, Samantha M., Rickman, John M., Pope, Nathaniel J., Noojin, Gary D., Scully, Marlan O., Denton, Michael L., Yakovlev, Vladislav V. |
المصدر: |
Biomedical Optics Express; November 2021, Vol. 12 Issue: 11 p7082-7091, 10p |
مستخلص: |
The electronic or molecular mechanisms that initiate photobiomodulation (PBM) in cells are not yet fully understood. The porcine complex III (C-III) of the electron transport chain was characterized with transient absorption spectroscopy (TAS). We then applied our recently developed continuous wave laser coupled TAS procedure (CW-TAS) to investigate the effect of red light irradiances on the heme dynamics of C-III in its c_1 reduced state. The time constants were found to be 3.3 ± 0.3 ps for vibrational cooling of the oxidized state and 4.9 ± 0.4 ps for rebinding of the photodissociated axial ligand of the c_1 reduced state. The analysis of the CW-TAS procedure yielded no significant changes in the C-III heme dynamics. We rule out the possibility of 635 nm CW light at 4.7 mW/cm^2 inducing a PBM effect on the heme dynamic of C-III, specifically with the photodissociation of its axial ligand. |
قاعدة البيانات: |
Supplemental Index |