التفاصيل البيبلوغرافية
| العنوان: |
A Purified Alkaline and Detergent-Tolerant Lipase from Aspergillus fumigatuswith Potential Application in Removal of Mustard Oil Stains from Cotton Fabric |
| المؤلفون: |
Mehta, Akshita, Gupta, Aashna, Bhardwaj, Kamal Kumar, Gupta, Reena |
| المصدر: |
Tenside Surfactants Detergents; November 2021, Vol. 58 Issue: 6 p442-451, 10p |
| مستخلص: |
In the present study, the lipase from Aspergillus fumigatuswas purified which was found to be stable to commercial detergents and oxidising agents. A purification fold of 6.96 and yield of 11.03% were achieved when the enzyme was purified using Octyl Sepharose column chromatography. In presence of various oxidizing agents, the highest activity of lipase was 15.56 U/mg with hydrogen peroxide. Among various surfactants used, the maximum activity exhibited by lipase was with Tween 80. While studying the effect of various detergents, the highest activity of 9.3 U/mg was achieved with "Vanish" detergent. Wash performance was studied with various detergents out of which "Vanish" showed highest oil removal of 79%. Lipase from Aspergillus fumigatuspossessed better stability with various surfactants and oxidizing agents. The results of this study have shown that the lipase from Aspergillus fumigatusalong with detergent "Vanish" (0.7%) under optimized conditions (5 μg/ml lipase, 40°C wash temperature and 40 min wash duration) improved oil removal from cotton fabric stained with mustard oil by 84%. |
| قاعدة البيانات: |
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