دورية
In vitro exocytosis in sea urchin eggs requires a synaptobrevin-related protein
| العنوان: | In vitro exocytosis in sea urchin eggs requires a synaptobrevin-related protein |
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| المؤلفون: | Avery, Julia, Hodel, Alois, Whitaker, Michael |
| المصدر: | Journal of Cell Science; July 1997, Vol. 110 Issue: 14 p1555-1561, 7p |
| مستخلص: | Sea urchin eggs provide an efficient in vitro model of exocytosis. We have identified proteins in sea urchin eggs that cross-react with antibodies to mammalian synaptobrevin, synaptotagmin, SNAP-25, syntaxin and rab3a. We show that these proteins are localized to the sea urchin egg cortex, using western blotting and immunocytochemistry. Tetanus toxin light chain cleaves the synaptobrevin-related protein in vitro and inhibits calcium-induced exocytosis. These data demonstrate a conservation between phyla of protein sequence and molecular mechanisms thought to facilitate exocytosis and show that the sea urchin egg provides a unique in vitro exocytotic model with which to study the conserved protein machinery of membrane fusion during secretion. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00219533 14779137 |
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| DOI: | 10.1242/jcs.110.14.1555 |