دورية
Optimization of Electroporation Conditions for Bacillus pumilus3–19 Strain
| العنوان: | Optimization of Electroporation Conditions for Bacillus pumilus3–19 Strain |
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| المؤلفون: | Danilova, I. V., Rudakova, N. L., Vasilyeva, Y. A., Gilmutdinova, A. I., Diadkina, I. V., Khasanov, D. I., Sharipova, M. R. |
| المصدر: | BioNanoScience; 20220101, Issue: Preprints p1-5, 5p |
| مستخلص: | Electroporation is the process of using electrical impulses to create temporary pores in the plasma membrane which, in turn, enables the penetration of nucleic acids into the cytoplasm of a cell. This method is widely used for the rapid and efficient introduction of foreign DNA into a wide range of cells. Cell viability and electrotransfection efficiency depend on various experimental factors, including the impulse form, vector concentration, cell type (density), properties of the electroporation buffer, and the growth phase of the bacterial culture. In this work, we investigated the optimal conditions for the transformation of the Bacillus pumilus3–19 strain using electroporation. Competent B. pumiluscells were obtained on the 4th hour of culture growth using PEB1 electroporation buffer and SOC1 (Super Optimal broth with Catabolic repressor) medium, while the electric field strength was 12 kV/cm. With these parameters, the transformation efficiency of bacillus cells was 56.3 transformants/μg DNA. Thus, the rational choice of pulsation conditions and buffering compositions is critical for the design of electroporation protocols to maximize the viability and efficiency of electrotransfection. |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21911630 21911649 |
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| DOI: | 10.1007/s12668-022-00982-2 |