دورية
Revisiting dextran effect on red blood cell to understand the importance of rouleaux distribution and red blood cell-endothelial cell adhesion
| العنوان: | Revisiting dextran effect on red blood cell to understand the importance of rouleaux distribution and red blood cell-endothelial cell adhesion |
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| المؤلفون: | Vijayaraghavan, Mahalakshmi, Chatterjee, Suvro, Sumantran, V. N., Jayavelu, Tamilselvan |
| المصدر: | Biomass Conversion and Biorefinery; 20240101, Issue: Preprints p1-11, 11p |
| مستخلص: | Biopolymers are naturally synthesized from algae, fungi, and plants. One among them is dextran, which has been extensively studied and still being explored to clarify its mechanistic function in supporting red blood cell (RBC) aggregation. Although its importance is widely established, in our present study, we have reexamined RBC aggregation stability by evaluating distribution of RBC rouleaux shape and its adhesive nature towards endothelial cell (EC) in a non-flowing environment. Our observation on aggregated RBC, besides showing increment in rouleaux number, also revealed increment in rouleaux size by exhibiting constant rouleaux shapes namely I-shape, L-shape, Y-shape, and clump-shaped with gradient change in dextran concentration (450–650 kDa). In addition, our study also highlights that aside from stimulating aggregation in RBC, dextran (7.5 and 10% DEX) consistently promotes single RBC adhesion towards EC than aggregated RBCs. Overall, our present study demonstrates rouleaux shape distribution and single RBC adhesion to EC as an important causative factor in provoking abnormalities. From the obtained data, we postulate that dextran-RBC interactions must be considered when designing biomaterials with dextran biopolymer for clinical applications. |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21906815 21906823 |
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| DOI: | 10.1007/s13399-022-02851-0 |