دورية
Developmental and hormonal regulation of alpha 2u-globulin gene transcription.
| العنوان: | Developmental and hormonal regulation of alpha 2u-globulin gene transcription. |
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| المؤلفون: | Kulkarni, A B, Gubits, R M, Feigelson, P |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America; May 1985, Vol. 82 Issue: 9 p2579-2582, 4p |
| مستخلص: | Hepatic alpha 2u-globulin protein and RNA levels are under developmental and complex multihormonal control. The present studies directly evaluate the degree to which this regulation is transcriptional. alpha 2u-Globulin transcription was determined by measuring nuclear runoff RNA in vitro, and tissue alpha 2u-globulin mRNA levels were measured by dot blot hybridization. These studies reveal that (i) in male rats the transcriptional rate of the alpha 2u-globulin genes increases during postnatal development; (ii) no alpha 2u-globulin transcription is detectable in hepatic nuclei derived from hypophysectomized rats; (iii) growth hormone and glucocorticoid are both absolutely required, and glucocorticoid can replace androgen for alpha 2u-globulin gene transcription in the livers of hypophysectomized male rats; and (iv) chronic treatment of mature male rats with estrogen results in a progressive decrease in the hepatic transcription of alpha 2u-globulin genes. In all instances changes in the transcriptional rate of alpha 2u-globulin genes paralleled the tissue level of alpha 2u-globulin RNA. Thus transcriptional control predominates in regulating hepatic alpha 2u-globulin RNA levels. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.82.9.2579 |