دورية
Rapid similarity searches of nucleic acid and protein data banks.
| العنوان: | Rapid similarity searches of nucleic acid and protein data banks. |
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| المؤلفون: | Wilbur, W J, Lipman, D J |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America; February 1983, Vol. 80 Issue: 3 p726-730, 5p |
| مستخلص: | With the development of large data banks of protein and nucleic acid sequences, the need for efficient methods of searching such banks for sequences similar to a given sequence has become evident. We present an algorithm for the global comparison of sequences based on matching k-tuples of sequence elements for a fixed k. The method results in substantial reduction in the time required to search a data bank when compared with prior techniques of similarity analysis, with minimal loss in sensitivity. The algorithm has also been adapted, in a separate implementation, to produce rigorous sequence alignments. Currently, using the DEC KL-10 system, we can compare all sequences in the entire Protein Data Bank of the National Biomedical Research Foundation with a 350-residue query sequence in less than 3 min and carry out a similar analysis with a 500-base query sequence against all eukaryotic sequences in the Los Alamos Nucleic Acid Data Base in less than 2 min. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.80.3.726 |