دورية
Two promoters integrate multiple enhancer inputs to drive wild-type knirpsexpression in the Drosophila melanogasterembryo
| العنوان: | Two promoters integrate multiple enhancer inputs to drive wild-type knirpsexpression in the Drosophila melanogasterembryo |
|---|---|
| المؤلفون: | Li, Lily, Waymack, Rachel, Gad, Mario, Wunderlich, Zeba |
| المصدر: | Genetics; December 2021, Vol. 219 Issue: 4 |
| مستخلص: | Proper development depends on precise spatiotemporal gene expression patterns. Most developmental genes are regulated by multiple enhancers and often by multiple core promoters that generate similar transcripts. We hypothesize that multiple promoters may be required either because enhancers prefer a specific promoter or because multiple promoters serve as a redundancy mechanism. To test these hypotheses, we studied the expression of the knirpslocus in the early Drosophila melanogasterembryo, which is mediated by multiple enhancers and core promoters. We found that one of these promoters resembles a typical “sharp” developmental promoter, while the other resembles a “broad” promoter usually associated with housekeeping genes. Using synthetic reporter constructs, we found that some, but not all, enhancers in the locus show a preference for one promoter, indicating that promoters provide both redundancy and specificity. By analyzing the reporter dynamics, we identified specific burst properties during the transcription process, namely burst size and frequency, that are most strongly tuned by the combination of promoter and enhancer. Using locus-sized reporters, we discovered that enhancers with no promoter preference in a synthetic setting have a preference in the locus context. Our results suggest that the presence of multiple promoters in a locus is due both to enhancer preference and a need for redundancy and that “broad” promoters with dispersed transcription start sites are common among developmental genes. They also imply that it can be difficult to extrapolate expression measurements from synthetic reporters to the locus context, where other variables shape a gene’s overall expression pattern. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00166731 19432631 |
|---|---|
| DOI: | 10.1093/genetics/iyab154 |