دورية
أعرض في EDS

Genome-wide characterization of Ficus caricaMADS-box transcription factors with a focus on their roles during fruit development

التفاصيل البيبلوغرافية
العنوان: Genome-wide characterization of Ficus caricaMADS-box transcription factors with a focus on their roles during fruit development
المؤلفون: Kmeli, Narjes, Hamdi, Jihen, Bouktila, Dhia
المصدر: Horticulture, Environment, and Biotechnology; April 2023, Vol. 64 Issue: 2 p311-329, 19p
مستخلص: MCM1-AGAMOUS-DEFICIENS-SRF (MADS)-box transcription factors (TFs) regulate a variety of plant developmental processes, particularly floral organ identity and fruit ripening. However, little is known about the MADS-box TF family in the common fig (Ficus caricaL.), a vital fruit crop of Mediterranean countries. Here, we report a comprehensive overview of the MADS-box genes and their TF products in fig, describing their classification, physicochemical properties, protein and gene architectures, phylogenetic relationships, selection mode and differential expression during fruit development. A total of 64 MADS-box members were identified in F. caricaand phylogenetically categorized as either type I (30) or type II (34). Type I MADS-box TFs were divided into three families (Mα, Mβ and Mγ, with 16, 4 and 10 members, respectively), whereas type II TFs were classified into two families (MIKCCand MIKC*, with 29 and 5 members, respectively). MIKCCTFs could be further classified into 12 subfamilies. Most FcMADS genes within the same clade were characterized by similar exon–intron organizations and motif compositions. Comparative phylogenetic analysis using mulberry (Morus notabilis) identified 24 (18 type II and 6 type I) orthologs between F. caricaand M. notabilis. In addition, 11 paralogous MADS-box gene pairs were identified in F. carica, which evolved under purifying selection, except for two recent paralogs from the TM3 (SOC1) subfamily. RNA-seq results indicated that 28 and 34 FcMADSgenes were differentially expressed in fruit peel and female flowers, respectively, during six successive stages of fruit development. According to their expression profiles, genes were grouped into four clusters (I, II, III and IV) in both tissues. FcMADSgenes from fruit peel expression cluster IV (FcMADS13, -23, -32, -40and -60) and female flower expression cluster III (FcMADS9, -49and -58) were upregulated during fruit ripening in the corresponding tissues, suggesting a potential, tissue-specific role of these candidate genes in fruit ripening. Our findings provide the first genome-wide extended characterization of the MADS-box TF family in F. carica, laying the groundwork for future research on its molecular roles in fruit ripening.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:22113452
22113460
DOI:10.1007/s13580-022-00478-8