دورية
Genotypic and Phenotypic Characterization of SalmonellaIsolated from Fresh Ground Meats Obtained from Retail Grocery Stores in the Brookings, South Dakota, Area
| العنوان: | Genotypic and Phenotypic Characterization of SalmonellaIsolated from Fresh Ground Meats Obtained from Retail Grocery Stores in the Brookings, South Dakota, Area |
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| المؤلفون: | Erickson, Alan K., Murray, Debra L., Ruesch, Laura A., Thomas, Milton, Lau, Zachary, Scaria, Joy |
| المصدر: | Journal of Food Protection; September 2018, Vol. 81 Issue: 9 p1526-1534, 9p |
| مستخلص: | Salmonellais one of the most common foodborne pathogens found in retail fresh meat products. The purpose of this study was to characterize the Salmonellathat is found in common types of fresh ground meats available to consumers in grocery stores in the Brookings, South Dakota, area. Salmonellaserotypes were detected in 50 (19%) of 261 retail fresh ground meat samples, with 2 (2%) of 115 ground turkey samples, 6 (14%) of 42 chicken samples, and 42 (40%) of 104 ground pork samples testing positive for Salmonella. The Salmonellaisolates were sequenced using an Illumina MiSeq genome sequencer. The resulting genomic sequences were analyzed to determine the serotypes of the isolates and to detect the presence of virulence and antibiotic resistance genes. The Salmonellaisolated from the ground meats belonged to 23 different serotypes. The predominant serotype isolated from ground chicken was Enteriditis (5 of 6, 83%). Among the ground pork isolates, the most common serotypes were the potential monophasic variant of Typhimurium (5 of 42, 12%), Uganda (5 of 42, 12%), Anatum (4 of 42, 10%), Derby (3 of 42, 7%), Infantis (3 of 42, 7%), and London (3 of 42, 7%). Among the 45 Salmonellaisolates tested to determine their resistance to common veterinary antibiotics, 25 (56%) were found to be susceptible to all 14 antibiotics tested, 11 (24%) were resistant to 1 antibiotic, 4 (9%) were resistant to 2 antibiotics, 1 (2%) was resistant to 3 antibiotics, 2 (4%) were resistant to 4 antibiotics, 1 (2%) was resistant to 8 antibiotics, and 1 (2%) was resistant to 10 antibiotics. The most common antibiotic resistances observed in this study were to streptomycin (15 of 45, 33%), tetracycline (11 of 45, 24%), and sulfisoxazole (7 of 45, 16%). The results of phenotypic evaluation of antibiotic resistance profiles of Salmonellaisolates correlated well with the antibiotic resistance genes detected in the genomic sequences of the isolates. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0362028X 19449097 |
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| DOI: | 10.4315/0362-028X.JFP-18-076 |