دورية
Bacteriological Survey of Fresh Minced Beef on Sale at Retail Outlets in Scotland in 2019: Three Foodborne Pathogens, Hygiene Process Indicators, and Phenotypic Antimicrobial Resistance
| العنوان: | Bacteriological Survey of Fresh Minced Beef on Sale at Retail Outlets in Scotland in 2019: Three Foodborne Pathogens, Hygiene Process Indicators, and Phenotypic Antimicrobial Resistance |
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| المؤلفون: | Bishop, H., Evans, J., Eze, J.I., Webster, C., Humphry, R.W., Beattie, R., White, J., Couper, J., Allison, L., Brown, D., Tongue, S.C. |
| المصدر: | Journal of Food Protection; September 2022, Vol. 85 Issue: 9 p1370-1379, 10p |
| مستخلص: | The health and economic burden of foodborne illness is high, with approximately 2.4 million cases occurring annually in the United Kingdom. A survey to understand the baseline microbial quality and prevalence of food-related hazards of fresh beef mince on retail sale could inform risk assessment, management, and communication to ensure the safety of this commodity. In such a survey, a two-stage sampling design was used to reflect variations in population density and the market share of five categories of retail outlets in Scotland. From January to December 2019, 1,009 fresh minced beef samples were collected from 15 geographic areas. The microbial quality of each sample was assessed using aerobic colony count and Escherichia colicount. Samples were cultured for Campylobacterand Salmonella,and PCR was used to detect target genes (stx1all variants, stx2a to g, and rfbO157) for Shiga toxin–producing E. coli(STEC). The presence of viable E. coliO157 and STEC in samples with a positive PCR signal was confirmed via culture and isolation. Phenotypic antimicrobial sensitivity patterns of cultured pathogens and 100 E. coliisolates were determined, mostly via disk diffusion. The median aerobic colony count and E. colicounts were 6.4 × 105(interquartile range, 6.9 × 104to 9.6 × 106) and <10 CFU/g (interquartile range, <10 to 10) of minced beef, respectively. The prevalence was 0.1% (95% confidence interval [CI], 0 to 0.7%) for Campylobacter,0.3% (95% CI, 0 to 1%) for Salmonella,22% (95% CI, 20 to 25%) for PCR-positive STEC, and 4% (95% CI, 2 to 5%) for culture-positive STEC. The evidence for phenotypic antimicrobial resistance detected did not give cause for concern, mainly occurring in a few E. coliisolates as single nonsusceptibilities to first-line active substances. The low prevalence of pathogens and phenotypic antimicrobial resistance is encouraging, but ongoing consumer food safety education is necessary to mitigate the residual public health risk. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0362028X 19449097 |
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| DOI: | 10.4315/JFP-22-051 |