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Biodegradation characteristics and genomic analysis of a newly isolated indole-degrading strain Pseudomonas aeruginosaJade-X

التفاصيل البيبلوغرافية
العنوان: Biodegradation characteristics and genomic analysis of a newly isolated indole-degrading strain Pseudomonas aeruginosaJade-X
المؤلفون: Zhang, Jiaxin, Ma, Qiao, Wang, Caihong, Lu, Shuxian, Fan, Shengqiang
المصدر: International Microbiology; 20230101, Issue: Preprints p1-9, 9p
مستخلص: Indole is a typical heterocyclic compound derived from tryptophan widespread in nature. Pseudomonas aeruginosais one of the most common opportunistic pathogens everywhere in the world. Indole and P. aeruginosawill encounter inevitably; however, the indole transformation process by P. aeruginosaremains unclear. Herein, an indole-degrading strain of P. aeruginosaJade-X was isolated from activated sludge. Strain Jade-X could degrade 1 mmol/L indole within 48 h with the inoculum size of 1% (v/v). It showed high efficiency in indole degradation under the conditions of 30–42 °C, pH 5.0–9.0, and NaCl concentration less than 2.5%. The complete genome of strain Jade-X was sequenced which was 6508614 bp in length with one chromosome. Bioinformatic analyses showed that strain Jade-X did not contain the indole oxygenase gene. Three cytochrome P450 genes were identified and up-regulated in the indole degradation process by RT-qPCR analysis, while cytochrome P450 inhibitors did not affect the indole degradation process. It suggested that indole oxidation was catalyzed by an unraveled enzyme. An antgene cluster was identified, among which the anthranilate 1,2-dioxygenase and catechol 1,2-dioxygenase genes were upregulated. An indole-anthranilate-catechol pathway was proposed in indole degradation by strain P. aeruginosaJade-X. This study enriched our understanding of the indole biodegradation process in P. aeruginosa.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:11396709
16181905
DOI:10.1007/s10123-023-00408-3