دورية
Understanding the Multi-Functional Role of TCTP in the Regeneration Process of Earthworm, Perionyx excavatus
| العنوان: | Understanding the Multi-Functional Role of TCTP in the Regeneration Process of Earthworm, Perionyx excavatus |
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| المؤلفون: | Rajagopalan, Kamarajan, Christyraj, Jackson Durairaj Selvan, Chelladurai, Karthikeyan Subbiahanadar, Das, Puja, Mahendran, Karthikeyan, Nagarajan, Logeshwari, Gunalan, Saritha |
| المصدر: | Tissue Engineering and Regenerative Medicine; 20230101, Issue: Preprints p1-14, 14p |
| مستخلص: | Background:: Regeneration is a highly complex process that requires the coordination of numerous molecular events, and identifying the key ruler that governs is important to investigate. While it has been shown that TCTP is a multi-functional protein that regulates cell proliferation, differentiation, apoptosis, anti-apoptosis, stem cell maintenance, and immune responses, but only a few studies associated to regeneration have been reported. To investigate the multi-functional role of TCTP in regeneration, the earthworm Perionyx excavatuswas chosen. Methods:: Through pharmacological suppression of TCTP, amputation, histology, molecular docking, and western blotting, the multi-function role of TCTP involved in regeneration is revealed. Results:: Amputational studies show that P. excavatusis a clitellum-independent regenerating earthworm resulting in two functional worms upon amputation. Arresting cell cycle at the G1/S boundary using 2 mM Thymidine confirms that P. excavatusexecute both epimorphosis and morphallaxis regeneration mode. The pharmacological suppression of TCTP using buclizine results in regeneration suppression. Following the combinatorial injection of 2 mM Thymidine and buclizine, the earthworm regeneration is completely blocked, which suggests a critical functional role of TCTP in morphallaxis. The pharmacological inhibition of TCTP also suppresses the key proteins involved in regeneration: Wnt3a (stem cell marker), PCNA (cell proliferation) and YAP1 (Hippo signalling) but augments the expression of cellular stress protein p53. Conclusion:: The collective results indicate that TCTP synchronously is involved in the process of stem cell activation, cell proliferation, morphallaxis, and organ development in the regeneration event. |
| قاعدة البيانات: | Supplemental Index |
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Full Text Finder | تدمد: | 17382696 22125469 |
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| DOI: | 10.1007/s13770-023-00599-w |