دورية
Antibiotic alters host’s gut microbiota, fertility, and antimicrobial peptide gene expression vis-à-visampicillin treatment on model organism Drosophila melanogaster
| العنوان: | Antibiotic alters host’s gut microbiota, fertility, and antimicrobial peptide gene expression vis-à-visampicillin treatment on model organism Drosophila melanogaster |
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| المؤلفون: | Singh, Asem Sanjit, Pathak, Dhruv, Devi, Manoharmayum Shaya, Anifowoshe, Abass Toba, Nongthomba, Upendra |
| المصدر: | International Microbiology; 20240101, Issue: Preprints p1-12, 12p |
| مستخلص: | Antibiotics are commonly used to treat infectious diseases; however, persistence is often expressed by the pathogenic bacteria and their long-term relative effect on the host have been neglected. The present study investigated the impact of antibiotics in gut microbiota (GM) and metabolism of host. The effect of ampicillin antibiotics on GM of Drosophila melanogasterwas analyzed through deep sequencing of 16S rRNA amplicon gene. The dominant phyla consisted of Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Planctomycetes, Chloroflexi, Euryarchaeota, Acedobacteria, Verrucomicrobia, and Cyanobacteria. It was found that the composition of GM was significantly altered on administration of antibiotics. On antibiotic treatments, there were decline in relative abundance of Proteobacteria and Firmicutes, while there were increase in relative abundance of Chlorophyta and Bacteroidota. High abundance of 14 genera, viz., Wolbachia, Lactobacillus, Bacillus, Pseudomonas, Thiolamprovum, Pseudoalteromonas, Vibrio, Romboutsia, Staphylococcus, Alteromonas, Clostridium, Lysinibacillus, Litoricola, and Cellulophagawere significant (p≤ 0.05) upon antibiotic treatment. Particularly, the abundance of Acetobacterwas significantly (p≤ 0.05) declined but increased for Wolbachia. Further, a significant (p≤ 0.05) increase in Wolbachia endosymbiont of D. melanogaster, Wolbachia endosymbiontof Curculio okumai, and Wolbachia pipientisand a decrease in the Acinetobactersp. were observed. We observed an increase in functional capacity for biosynthesis of certain nucleotides and the enzyme activities. Further, the decrease in antimicrobial peptide production in the treated group and potential effects on the host’s defense mechanisms were observed. This study helps shed light on an often-overlooked dimension, namely the persistence of antibiotics’ effects on the host. |
| قاعدة البيانات: | Supplemental Index |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 11396709 16181905 |
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| DOI: | 10.1007/s10123-024-00507-9 |