دورية
Ultrastructural and Functional Studies of the Interaction between IL-12 and IL-2 for the Generation of Lymphokine-Activated Killer Cells
| العنوان: | Ultrastructural and Functional Studies of the Interaction between IL-12 and IL-2 for the Generation of Lymphokine-Activated Killer Cells |
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| المؤلفون: | Facchetti, Paola, Tacchetti, Carlo, Prigione, Ignazia, Airoldi, Irma, Favre, Anna, Grossi, Carlo Enrico, Pistoia, Vito |
| المصدر: | Experimental Cell Research; December 1999, Vol. 253 Issue: 2 p440-453, 14p |
| مستخلص: | IL-12 promotes generation of LAK activity in short-term-cultured NK cells, but information on the structure and function of IL-12-induced LAK cells is not yet available. The latter issues have been here investigated with emphasis on interactions between IL-12 and IL-2. Peripheral blood mononuclear cells (MNC) exposed to IL-12 for 5–7 days displayed a decrease in the amount and density of the matrix of large granular lymphocyte (LGL)-associated granules. In cells cultured with IL-12 and IL-2 for 5–7 days, empty vacuoles were predominant and the electron-dense matrix was scanty. In MNC incubated with IL-2 for 5–7 days, most granules were loaded with electron-dense matrix. IL-12 and IL-2 displayed an additive effect on LAK cell cytotoxicity until approximately 48 h in culture which was followed by a sharp decline. Immunocytochemical and biochemical studies demonstrated that MNC cultured for 5–7 days with IL-12 and IL-2 displayed downregulated perforin expression and upregulated granzyme B expression. Fas ligand expression was virtually undetectable in MNC cultured for 5–7 days with or without cytokines. It appears that perforin downregulation plays a major role in the reduced cytotoxicity of MNC cultured with IL-12 and IL-2 for 5–7 days. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00144827 10902422 |
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| DOI: | 10.1006/excr.1999.4697 |