دورية
Detection of virulent Klebsiella pneumoniaestrains causing intestinal and extraintestinal infections during the 80s and 90s in Brazil
| العنوان: | Detection of virulent Klebsiella pneumoniaestrains causing intestinal and extraintestinal infections during the 80s and 90s in Brazil |
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| المؤلفون: | Valiatti, Tiago Barcelos, Nunes, Pedro Henrique Soares, Santos, Fernanda Fernandes, Cayô, Rodrigo, Marcelino, Ingrid Nayara, Alberto-Lei, Felipe, Varjão, Haian Araujo, Gales, Ana Cristina, Gomes, Tânia Aparecida Tardelli |
| المصدر: | Brazilian Journal of Microbiology; 20240101, Issue: Preprints p1-9, 9p |
| مستخلص: | Klebsiella pneumoniaeis an important pathogen that causes several human infections, which is currently among the main bacterial species of clinical importance. Given the importance of understanding the characteristics of this pathogen and its evolutionary aspects, in this study, we sought to characterize strains of K. pneumoniaerecovered in the 1980s and 1990s in São Paulo, Brazil. Our analyses included 48 strains recovered from diarrheagenic stools and extraintestinal infections. These strains were submitted to screening for virulence and ESβL-encoding genes, antimicrobial susceptibility tests, biofilm formation, and hypermucosity and hemolytic activity tests. Our results revealed that among the studied virulence genes, the most frequent were entB(100%), followed by iutA(100%), mrkD(98%), and ycfM(72%). Phenotypic tests revealed that the strains were non- hemolytic, and two strains were positive for the hypermucoviscosity phenotype but did not have the genetic markers associated with this phenotype. Furthermore, 17% of the isolates proved to be strong biofilm producers. Antimicrobial susceptibility testing demonstrated that most strains were susceptible to the tested antimicrobials, with the exception of five isolates that produced CTX-M-2. Our findings indicate that the collection of strains studied showed variability in virulence factors, as well as biofilm production. Still, a minority of the strains showed clinically significant resistance mechanisms. As far as we know, this is the oldest collection of K. pneumoniaestudied in the country. |
| قاعدة البيانات: | Supplemental Index |
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Full Text Finder | تدمد: | 15178382 16784405 |
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| DOI: | 10.1007/s42770-024-01502-y |