دورية
Bioactivation of 5-Hydroxymethyl-2-Furaldehyde to an Electrophilic and Mutagenic Allylic Sulfuric Acid Ester
| العنوان: | Bioactivation of 5-Hydroxymethyl-2-Furaldehyde to an Electrophilic and Mutagenic Allylic Sulfuric Acid Ester |
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| المؤلفون: | Lee, Y.C., Shlyankevich, M., Jeong, H.K., Douglas, J.S., Surh, Y.J. |
| المصدر: | Biochemical and Biophysical Research Communications; April 1995, Vol. 209 Issue: 3 p996-1002, 7p |
| مستخلص: | 5-Hydroxymethyl-2-furaldehyde (HMF), a ubiquitous food contaminant, has been proposed to be metabolically activated through sulfonation of its allylic hydroxyl functional group. In support of this idea, we have found the strong direct mutagenicity of chemically synthesized sulfuric acid ester, 5-sulfooxymethylfurfural (SMF), in Salmonella typhimuriumTA104. The intrinsic mutagenicity of this reactive ester was significantly inhibited by glutathione and glutathione S-transferase activity in dialyzed rat liver cytosol. The metabolic formation of SMF was elucidated by enhanced mutagenicity of HMF in the presence of rat hepatic cytosol enriched with the sulfogroup donor, 3′-phosphoadenosine-5′-phosphosulfate (PAPS). The PAPS- and cytosol-dependent mutagenicity of HMF was markedly lessened by sulfotransferase inhibitors such as 2,6-dichloro-4-nitrophenol and dehydroepiandrosterone. These results suggest that HMF can be metabolically activated to an allylic sulfuric acid ester which may play a role as an ultimate electrophilic metabolite in toxification of the parent compound in vivo. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0006291X 10902104 |
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| DOI: | 10.1006/bbrc.1995.1596 |