دورية
Modification of a Synthetic Antimicrobial Peptide (ESF1) for Improved Inhibitory Activity
| العنوان: | Modification of a Synthetic Antimicrobial Peptide (ESF1) for Improved Inhibitory Activity |
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| المؤلفون: | Dykes, Gary A., Aimoto, Saburo, Hastings, John W. |
| المصدر: | Biochemical and Biophysical Research Communications; July 1998, Vol. 248 Issue: 2 p268-272, 5p |
| مستخلص: | Rational modification of an existing cationic α-helical antimicrobial peptide (ESF1) for improved activity by increasing amphipathicity was undertaken. ESF1 and two variants (GR7 and SA3) were synthesized and tested for activity range, minimum inhibitory concentration, and hemolytic activity. Biological activity was related to structure as determined by circular dichroism. The substitution of arginine for glycine in position seven was found to increase antimicrobial activity without effecting hemolysis. Increased activity was related to stronger α-helix formation in buffer. Increased β-sheet formation in micellar SDS was observed and speculated to be due to a stronger ability of the variants to form multimolecule complexes, a feature consistent with existing models of cationic peptide activity. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0006291X 10902104 |
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| DOI: | 10.1006/bbrc.1998.8940 |