دورية
Upregulation of the Apoptosis-Associated Protein Grb3-3 in HIV-1-Infected Human CD4+ Lymphocytes
| العنوان: | Upregulation of the Apoptosis-Associated Protein Grb3-3 in HIV-1-Infected Human CD4+ Lymphocytes |
|---|---|
| المؤلفون: | Li, Xuguang, Multon, Marie-Christine, Henin, Yvette, Schweighoffer, Fabien, Venot, Corinne, LaVecchio, Joyce, Josef, Juliana, Stuckert, Patricia, Mhashilkar, Abner, Tocque´, Bruno, Marasco, Wayne A. |
| المصدر: | Biochemical and Biophysical Research Communications; September 16, 2000, Vol. 276 Issue: 1 p362-370, 9p |
| مستخلص: | The mechanism(s) by which HIV-1 infection contributes to depletion of CD4+ T cell is not well understood. In this report, we investigated whether a recently identified isoform of growth factor receptor bound protein (Grb2), named Grb3-3, a signaling molecule that is associated with the MAP kinase pathway and with apoptosis could be involved. We find that Grb3-3 is markedly up-regulated following HIV-1 infection of CD4+ peripheral blood mononuclear cells undergoing apoptosis. Although IL-2 deprived CD4+ cells also undergo apoptosis to a similar extent, Grb3-3 upregulation is not detected under these experimental conditions. Transient overexpression of Grb3-3 in Jurkat T-cells also causes apoptosis. Upon staurosporine stimulation, Grb3-3 predisposes Sup-T1 cell to apoptosis. Finally, analysis of the HIV-1 genes responsible for Grb3-3 expression demonstrates that Tat and Nef can independently induces its expression, suggesting these two earliest viral gene products of HIV-1 may share some common pathway(s) in up-regulating Grb3-3 expression. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0006291X 10902104 |
|---|---|
| DOI: | 10.1006/bbrc.2000.3415 |