دورية
Effects of culture conditions on production of type 5 capsular polysaccharide by human and bovine Staphylococcus aureus strains.
| العنوان: | Effects of culture conditions on production of type 5 capsular polysaccharide by human and bovine Staphylococcus aureus strains. |
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| المؤلفون: | Poutrel, B, Gilbert, F B, Lebrun, M |
| المصدر: | Clinical and Vaccine Immunology (formerly CDLI); March 1995, Vol. 2 Issue: 2 p166-71, 6p |
| مستخلص: | Two Staphylococcus aureus strains, the prototype human Reynolds strain and a bovine isolate, were grown in different complex media and in a synthetic medium (D. Taylor and K. T. Holland, J. Appl. Bacteriol. 66:319-329, 1989) and compared for their ability to produce type 5 capsular polysaccharide. Cell-bound and cell-free type 5 capsular polysaccharide were measured by a new one-step competition enzyme-linked immunosorbent assay. The total production and the proportion of cell-bound type 5 capsular polysaccharide were dependent on the nature of the medium, the duration of the culture, and the strain. Both strains produced more type 5 capsular polysaccharide when cultivated in the synthetic medium than when cultivated in complex media. The best yield of type 5 capsular polysaccharide, about 300 micrograms/ml of medium, was obtained with strain Reynolds grown for 48 h with shaking in the synthetic broth containing glucose as a carbon source. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15566811 1556679X |
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