دورية
TaqMan-Based Detection of Trichomonas vaginalisDNA from Female Genital Specimens
| العنوان: | TaqMan-Based Detection of Trichomonas vaginalisDNA from Female Genital Specimens |
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| المؤلفون: | Jordan, Jeanne A., Lowery, Donna, Trucco, Massimo |
| المصدر: | Journal of Clinical Microbiology; November 2001, Vol. 39 Issue: 11 p3819-3822, 4p |
| مستخلص: | ABSTRACTA double-labeled fluorescent probe was designed and evaluated for detecting Trichomonas vaginalisDNA in a 5' nuclease (TaqMan) assay. The T. vaginalis-specific probe contains a 5'-fluorescein (5'-FAM) and a 3'-rhodamine (TAMRA) derivative. Female genital secretions were collected on Amplicor (Roche Molecular, Indianapolis, Ind.) swabs and by a transport system used forChlamydia trachomatisand/or Neisseria gonorrhoeaeDNA detection by PCR. Five hundred fifty-two female genital specimens, of which 248 (45%) were vaginal specimens and 304 (55%) were introital, were tested for both T. vaginalisDNA and viable microorganisms using the 5' nuclease assay and broth culture, respectively. Of these, 304 of 552 (55%) were also evaluated by direct microscopic examination for the characteristic motile organism. After resolving discrepancies, the comparisons produced an analytical sensitivity and specificity for the TaqMan-based PCR assay of 97.8 and 97.4%, respectively. As a result, ?RQ values (differences in fluorescence due to probe hybridization and resulting 5'-FAM cleavage from the specific PCR product) of =2.0 and =1.5 were established for T. vaginalis-positive and -negative cutoffs, respectively. ?RQ values between 1.5 and 2.0 were considered indeterminate. Overall findings revealed a high level of agreement between PCR and culture for detecting T. vaginalis. Potential benefits of the 5' nuclease assay include a greater sensitivity compared to direct microscopic examination and the ease of testing large numbers of clinical specimens in a significantly shorter turnaround time compared to culture. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00951137 1098660X |
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| DOI: | 10.1128/JCM.39.11.3819-3822.2001 |