دورية
Isolation and Characterization of Mouse Xrcc-1, a DNA Repair Gene Affecting Ligation
| العنوان: | Isolation and Characterization of Mouse Xrcc-1, a DNA Repair Gene Affecting Ligation |
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| المؤلفون: | Brookman, Kerry W., Tebbs, Robert S., Allen, Susan A., Tucker, James D., Swiger, Roy R., Lamerdin, Jane E., Carrano, Anthony V., Thompson, Larry H. |
| المصدر: | Genomics; July 1994, Vol. 22 Issue: 1 p180-188, 9p |
| مستخلص: | Human DNA repair gene XRCC1complements the strand-break rejoining defect in Chinese hamster mutant EM9 and encodes a protein that is apparently required for optimal activity of DNA ligase III. Toward the goal of producing transgenic mice that carry a mutation in the Xrcc-1locus, the murine homolog of XRCC1was cloned from both cosmid genomic and cDNA libraries. Upon transfection into EM9 cells, cosmids containing the functional mouse gene efficiently corrected (94-100%) the high sister-chromatid-exchange defect. Mouse Xrcc-1is 26 kb in length, contains 17 exons, and maps by metaphase in situhybridization to the 7A3-7B2 region of mouse chromosome 7. Isolated cDNA clones were highly truncated and were extended by anchored polymerase chain reactions. The 1893-bp open reading frame of mouse Xrcc-1encodes 631 amino acids, compared with 633 for the human homolog. The predicted mouse Xrcc-1 protein of 69.1 kDa and pIof 5.95 is 86% identical and 93% similar to human XRCC1. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 08887543 10898646 |
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| DOI: | 10.1006/geno.1994.1359 |