دورية
Optimization of Reaction Parameters and Cultivation Conditions for Biocatalytic Hydrogen Transfer Employing Overexpressed ADH‐‘A’ from Rhodococcus ruberDSM 44541 in E. coli
| العنوان: | Optimization of Reaction Parameters and Cultivation Conditions for Biocatalytic Hydrogen Transfer Employing Overexpressed ADH‐‘A’ from Rhodococcus ruberDSM 44541 in E. coli |
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| المؤلفون: | Edegger, K., Gruber, C. C., Faber, K., Hafner, A., Kroutil, W. |
| المصدر: | Engineering in Life Sciences; April 2006, Vol. 6 Issue: 2 p149-154, 6p |
| مستخلص: | The alcohol dehydrogenase ADH‐‘A’ from Rhodococcus ruberDSM 44541 represents a highly efficient catalyst for biocatalytic hydrogen transfer reactions. Starting from an exceedingly low level of active ADH‐‘A’ in Escherichia coli, the apparent specific activity of ADH‐‘A’ overexpressed in E. colicells could be drastically enhanced by a factor of 550 by optimizing the host and induction/growth conditions. The influence of reaction parameters like pH, cosubstrate (2‐propanol, acetone) concentration, substrate concentration temperature and additional cofactor on the apparent activity was investigated. In contrast to the purified enzyme, the pH optimum for oxidation and reduction were identical. Due to the employment of whole cells of E. coli/ADH‐‘A’ as catalyst lower operational stability was found. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 16180240 16182863 |
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| DOI: | 10.1002/elsc.200620902 |