دورية
ProteomIQ Blue, a Potent Post-stain for the Visualization and Subsequent Mass Spectrometry Based Identification of Fluorescent Stained Proteins on 2D-gels
| العنوان: | ProteomIQ Blue, a Potent Post-stain for the Visualization and Subsequent Mass Spectrometry Based Identification of Fluorescent Stained Proteins on 2D-gels |
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| المؤلفون: | Wijte, Dorien, L. de Jong, Ad, A. E. Mol, Marijke, L. M. van Baar, Ben, J. R. Heck, Albert |
| المصدر: | Journal of Proteome Research; August 2006, Vol. 5 Issue: 8 p2033-2038, 6p |
| مستخلص: | Manual spot excision for protein identification from fluorescent stained two-dimensional (2-D) gels is hard to accomplish. Here, we explore the use of ProteomIQ Blue as a post-stain method for the visualization of fluorescent stained/labeled proteins. We show that ProteomIQ Blue post-staining is almost as sensitive as staining with SYPRO Ruby or cyanine dyes alone. More than 90% of the protein spots that are stained with the fluorescent stains are still detectable with ProteomIQ Blue. In protein identification by mass spectrometry, ProteomIQ Blue post-stained spots provide high sensitivity and high protein sequence coverage of the peptide mass maps in both MALDI-TOF−MS and ESI−MS/MS analyses. In conclusion, post-staining of fluorescent stained gels with ProteomIQ Blue provides a facile and a powerful method to achieve quantitative protein analysis as well as protein identification in the same semianalytical gel without requiring sophisticated/expensive robotic equipment. |
| قاعدة البيانات: | Supplemental Index |
Find this issue from the American Chemical Society | تدمد: | 15353893 15353907 |
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| DOI: | 10.1021/pr060076f |