Deletion of GLX3 in Candida albicans affects temperature tolerance, biofilm formation, and virulence
| العنوان: | Deletion of GLX3 in Candida albicans affects temperature tolerance, biofilm formation, and virulence |
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| المؤلفون: | Cabello L, Gómez-Herreros E, Fernández-Pereira J, Maicas S, Martínez-Esparza MC, de Groot PWJ, Valentín E |
| المصدر: | FEMS YEAST RESEARCH r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname |
| بيانات النشر: | OXFORD UNIV PRESS, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Glx3 protein, C. albicans, cell wall, biofilms, candidiasis |
| الوصف: | Candida albicans is a predominant cause of fungal infections in mucosal tissues as well as life-threatening bloodstream infections in immunocompromised patients. Within the human body, C. albicans is mostly embedded in biofilms, which provides increased resistance to antifungal drugs. The glyoxalase Glx3 is an abundant proteomic component of the biofilm extracellular matrix. Here, we document phenotypic studies of a glx3 null mutant concerning its role in biofilm formation, filamentation, antifungal drug resistance, cell wall integrity, and virulence. First, consistent with its function as glyoxalase, the glx3 null mutant showed impaired growth on media containing glycerol as the carbon source and in the presence of low concentrations of hydrogen peroxide. Importantly, the glx3 mutant showed decreased fitness at 37°C and formed less biofilm as compared to WT and a reintegrant strain. At the permissive temperature of 28°C, the glx3 mutant showed impaired filamentation as well as increased sensitivity to Calcofluor white, Congo red, SDS, and zymolyase, indicating subtle alterations in wall architecture even though gross quantitative compositional changes were not detected. Interestingly, and consistent with its impaired filamentation, biofilm formation and growth at 37°C, the glx3 mutant is avirulent. Our results underline the role of Glx3 in fungal pathogenesis and the involvement of the fungal wall in this process. |
| تدمد: | 1567-1356 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=RECOLECTA___::753b6e438f1316cfd67f2fc780e8a1f6 https://fundanet.iislafe.san.gva.es/publicaciones/ProdCientif/PublicacionFrw.aspx?id=9512 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.RECOLECTA.....753b6e438f1316cfd67f2fc780e8a1f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15671356 |
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