Computer-assisted development of RP-HPLC methods for the analysis of plant bioactive compounds
| العنوان: | Computer-assisted development of RP-HPLC methods for the analysis of plant bioactive compounds |
|---|---|
| المؤلفون: | Danilo Corradini, Isabella Nicoletti |
| المصدر: | XXVII Congresso Nazionale della Divisione di Chimica Analitica della Società Chimica Italiana, pp. P064–P064, Bologna, 09/16/2018, 09/20/2018 info:cnr-pdr/source/autori:Danilo Corradini, Isabella Nicoletti/congresso_nome:XXVII Congresso Nazionale della Divisione di Chimica Analitica della Società Chimica Italiana/congresso_luogo:Bologna/congresso_data:09%2F16%2F2018, 09%2F20%2F2018/anno:2018/pagina_da:P064/pagina_a:P064/intervallo_pagine:P064–P064 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Design of Experiments, RP-HPLC, Plant secondary metabolites |
| الوصف: | The identification and quantification of phenolic compounds occurring in plants and plant-derived food products is performed by a variety of techniques, including high performance liquid chromatography in reversed phase separation mode (RP-HPLC), which can be considered the technique of choice. The optimization of the RP-HPLC methods is usually carried out by conventional trial-and-error approaches, requiring the screening of a variety of experimental conditions, which include column temperature, pH and composition of the mobile phases, and shape and duration of the gradient elution program. This communication describes and discusses the results of our studies conducted for the computer-assisted development of RP-HPLC methods for separation, identification and quantification of phenolic compounds extracted from plant tissues and plant-derived cosmetic and food product. The study has been conducted by a Design of Experiments (DoE) approach that allow the simultaneous optimization of gradient time (tG), column temperature (T) and binary eluent composition on the basis of retention times and peak areas of the analytes of interest, obtained in twelve different experiments. These experiments consist in the linear gradient separations of the investigated samples performed at two different gradient times and column temperatures, using the aqueous component of the mobile phase at three different pH values. The presentation describes the use of the above experimental data to construct 3-D resolution maps, which evaluate the influence of column temperature, mobile phase pH and composition, and gradient duration on the retention and resolution of a variety of analytes. In our study, 3-D resolution maps have been constructed using either mixtures of standard phenolic compounds or samples of these compounds extracted from plants tissues or plant derived products. The goal of our study has been to model the experimental conditions that allow the optimal selection of the mobile phase composition and gradient elution shape and duration, in order to improve peak resolution and to reduce the analysis time, using a limited number of experiments and, consequently, reduced amounts of expensive and environmentally harmful chemicals. Excellent correlation between simulated and experimental separations of phenolic compounds are demonstrated. The application of the above DoE approach to the separation, identification and quantification of phenolic compounds occurring in a variety of plants and plant-derived cosmetic, food, and dietary supplement are illustrated and discussed. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=cnr_________::e85507bf63b2b7c8b8e3a226f7d80b6b http://www.cnr.it/prodotto/i/397147 |
| رقم الأكسشن: | edsair.cnr...........e85507bf63b2b7c8b8e3a226f7d80b6b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |