| الوصف: |
Objective: This study aimed to investigate the renoprotective effects of paricalcitol, a synhetic vitamin D analog, through its possible roles on p38 MAPK and PI3K/Akt signaling pathways to prevent oxidative stress, inflammation and apoptosis during renal I/R. Materials and methods: Total 20 kidney tissues of sham (n = 6), subjected to renal I/R bilaterally for 45 min ischemia followed by 24 h reperfusion (n = 7) and paricalcitol (0.3 mu g/kg, ip) pretreated Wistar albino rats (n = 7) were used in this study. Interstitial inflammation and active caspase-3 expression were evaluated histologically. TNF-alpha, IL-1 beta, kidney injury molecule-1 (KIM-1), MDA and SOD activity in kidneys were analysed biochemically. Furthermore, activation of p38 MAPK, PI3K/Akt signaling pathways and NF kappa B p65 were evaluated by western blot. Results: Paricalcitol pretreatment significantly reduced interstitial inflammation during renal I/R, which was consistent with decreased tumor TNF-alpha, IL-1 beta, active caspase-3 and KIM-1 expression. Paricalcitol also reduced MDA level and attenuated the reduction of SOD activity in the kidney during I/R. Moreover, paricalcitol could suppress the p38 MAPK and NF kappa B p65, and also activate PI3K/Akt signaling pathway during renal I/R. Conclusion: All these findings indicate that paricalcitol may be an effective practical strategy to prevent renal I/R injury. C1 [Cavdar, Zahide; Ural, Cemre; Kocak, Ayse] Dokuz Eylul Univ, Hlth Sci Inst, Dept Mol Med, TR-35340 Izmir, Turkey. [Arslan, Sevki] Pamukkale Univ, Fac Sci, Dept Biol, Denizli, Turkey. [Ersan, Sibel] Izmir Tepecik Res & Training Hosp, Dept Nephrol, Izmir, Turkey. [Ozbal, Seda; Tatli, Merve] Dokuz Eylut Univ, Fac Med, Dept Histol & Embryol, Izmir, Turkey. [Dubova, Asti] Dokuz Eylul Univ, Hlth Sci Inst, Dept Lab Anim Sci, Izmir, Turkey. [Cavdar, Caner] Dokuz Eylul Univ, Fac Med, Dept Nephrol, Izmir, Turkey. |
