In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
العنوان: | In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens |
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المؤلفون: | Zimmermann,CEP, Machado,AK, Cadoná,FC, Jaques,JAS, Schlemmer,KB, Lautert,C, Cruz,IBM, Zanette,RA, Leal,DBR, Santurio,JM |
المصدر: | Revista Brasileira de Ciência Avícola, Volume: 16, Issue: 3, Pages: 307-312, Published: SEP 2014 Brazilian Journal of Poultry Science v.16 n.3 2014 Brazilian Journal of Poultry Science Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
بيانات النشر: | Fundação APINCO de Ciência e Tecnologia Avícolas, 2014. |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Aflatoxin B1, Broilers, PicoGreen, Lymphocytes, Reactive oxygen species |
الوصف: | The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes. |
وصف الملف: | text/html |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::6a7cc5cd9977ab4480890abcc637b470 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012&lng=en&tlng=en |
حقوق: | OPEN |
رقم الأكسشن: | edsair.dedup.wf.001..6a7cc5cd9977ab4480890abcc637b470 |
قاعدة البيانات: | OpenAIRE |
الوصف غير متاح. |